Figure 1

GISH patterns obtained on different Setaria species. (a) GISH was carried out using S. viridis-Q24 genomic DNA as probe hybridizing on the chromosome preparation of S. italica-Y1. (b) Metaphase of Qing 9 probed with S. viridis-Q24 total genomic DNA probe, two major spots were detected in the nucleolar organizing regions (arrows). (c) DAPI counterstained metaphase plate from S. adhaerans-W94. (d) The same metaphase plate was hybridized with Qing 9 genomic DNA (red). (e) The metaphase of Qing 9 was counterstained with DAPI. (f) The same metaphase hybridized with genomic DNA of S. adhaerans-W94 (red). (g-h) Genomic DNA of S. viridis-Q24 (g) and S. adhaerans-W94 (h) was applied to S. lachnea chromosomes respectively. (i) DAPI counterstained metaphase plate from S. lachnea. (j) The same metaphase hybridized with the total genomic DNA of S. grisebachii. (k) The metaphase of S. palmifolia was counterstained with DAPI. (l) The same metaphase plate was hybridized with the genomic DNA of S. plicata (red). (m) DAPI counterstained metaphase plate from S. parviflora-W79. (n) The same metaphase hybridized with S. glauca-W12 genomic DNA. (o-r) The metaphases of S. arenaria respectively hybridized with the genomic DNA of S. viridis-Q24 (o), S. adhaerans-W94 (p), S. grisebachii (q), and S. glauca-W12 (r). (s) Metaphase of S. palmifolia probed with S. viridis-Q24 total genomic DNA probe. (t) Metaphase plate from S. glauca-W12 hybridized with probe from S. grisebachii. Bar = 5 μm.