Figure 4
Example fluorescence micrographs for expression of pqn-21::gfp fusions. A-C. The head, mid-section and tail, respectively, of an L4 larva from strain UL3453 with gfp inserted after the pqn-21 start codon. D, E. The head and tail, respectively, of an L2 larva from strain UL3412 with gfp inserted before the pqn-21 stop codon. F, G. Adults from strain UL1131 with a plasmid based reporter gene fusion from [22] with pqn-21 tagged with gfp at the 5th exon. A-E The corresponding DIC photomicrograph is provided beneath the fluorescence micrograph. A-C captured at 200x, D, E captured at 400x, F, G captured at 100x magnification.