Figure 2

Genome map of the S. agalactiae strain 2-22 and genome comparisons with other GBS strains. (a) Circular genome map of S. agalactiae strain 2-22 showing gene positions and orientations. From the outside: circle 1: protein coding genes on the + and - strands; circle 2, in green: genes for rRNA and tRNA; circle 3, in red: pseudogenes; circle 4: %GC plot; circle 5: GC skew [(GC)/(G + C)]. (b) Genome sequence alignment with S. agalactiae strains A909 and NEM316. Genomic islands characterized in GBS human strains are indicated in red and numbered according to [26] while genomic islands specific to strain 2-22 are in violet. CDS are shown in blue and rDNA genes in green. (c) SNP distribution along the genome sequences of ST260-261 strains. The number of SNPs as determined by nucmer [40] (y axis) per 1000 nt in the ST260 strains 90-503 and SS1219 versus strain 2-22 is plotted according to the position of the corresponding fragment on the strain 2-22 chromosome (x axis). The goodness of fit to a uniform distribution was tested using a Chi square test on 36 class intervals of 50000 nt and differences in SNP distribution were found to be not significant (p = 0.20).