Figure 1
From: Long-range transcriptional regulation by the p110 CUX1 homeodomain protein on theENCODE array
Expression of CUX1 Recombinant Proteins. (A) Schematicrepresentation of CUX1 proteins with some of the functional domains: ID,inhibitory domain; CC, coiled-coil; CR1, CR2 and CR3, Cut repeat 1, 2and 3; HD homeodomain; CBD, calmodulin binding domain; Prot A, proteinA. The regions recognized by the 861 and 1300 antibodies are shown.(B) Hs578t cells were infected with a retroviral vector toestablish a population of cells stably expressing a recombinant p110CUX1 protein with two tags at its C-terminus, p110 CUX1-Tag2.A population stably carrying the empty vector was used as a control.Nuclear extracts were prepared from each population of cells andanalyzed by Western blot using the 861 and 1300 CUX1 antibodies.