Figure 3

Hippocampal region histology following TBI. Damage to the ipsilateral hippocampal region was demonstrated by FJB staining (A). FJB staining was absent in the contralateral hippocampal region (B). While the integrity of the hippocampus appears intact ipsilaterally (C), many activated microglia and macrophages were present as demonstrated by ED-1 immunostaining (D). There were no activated macrophages in the contralateral hippocampal region (E) and the anatomical structure appears intact (F). FJB: green; DAPI: blue; ED-1: red. Scale bars: 250 μm ( A,B), 125 μm ( C-F).