Table 2 Primers used in this study
From: Functional genomic analysis of bile salt resistance in Enterococcus faecium
    Primer |                 Sequence a |
|---|---|
delete_XmaI_gltK_up_F | 5′-CCCCCCGGGCCAAGCAGGTACGATTGGAT-3′ |
delete_EcoRI_gltK_up_R | 5′-AACCGGAAAGCAGAGAATTCTCGAAAACAATGAAACTTCAACA-3′ |
delete_EcoRI_gltK_dn_F | 5′-TCGAGAATTCTCTGCTTTCCGGTTACTTGG-3′ |
delete_XhoI_gltK_dn_R | 5′-CCGCTCGAGGGAAGGATCACACCGATGAC-3′ |
check_gltK_up | 5′-CGGAACGTTAATGGCAATCT-3′ |
check_gltK_dn | 5′-CCGTACCAATCGTACCGATAA-3′ |
delete_XmaI_gspA_up_F | 5′-CCCCCCGGGCCTCCTTTTTGGACTTTCTCG-3′ |
delete_EcoRI_gspA_up_R | 5′-ACCACATTTAGCTGCAGAATTCGACGGCTTTCCGTTGTGTAG-3′ |
delete_EcoRI_gspA_dn_F | 5′-CGAATTCTGCAGCTAAATGTGGTACGAA-3′ |
delete_XhoI_gspA_dn_R | 5′-CCGCTCGAGGCCAAGTGAAAGCTTTGGAA-3′ |
check_gspA_up | 5′-GCTCGAATTCTTCGATTGCT-3′ |
check_gspA_dn | 5′-TGATGAGCCGTTAAATGGAA-3′ |
complement_BamHI_gltK_F | 5′-ACGGGATCCTTTTAGCAATCGTAGCTGGTTT-3′ |
complement_XhoI_gltK_R | 5′-ACCGCTCGAGCGTGAATTTTCAAGTGCTC-3′ |
complement_BamHI_gspA_F | 5′-ACGGGATCCTGAAAAACCTTCGATCGTTCA-3′ |
complement_XhoI_gspA_R | 5′-ACCGCTCGAGTCCATTCCTACTCCCCCTCT-3′ |
pAT392_EcoRI_lox66_genta_F | 5′-AGGGAATTCTACCGTTCGTATAGCATACATTATACGAAGTTATG ATAAACCCAGCGAACCATTTGAGG-3′ |
pAT392_EcoRI_lox71_genta_R | 5′-CTCCGAATTCTACCGTTCGTATAATGTATGCTATACGAAGTTATT CAATCTTTATAAGTCCTTTTATAA-3′ |