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Table 2 Primers used in this study

From: Functional genomic analysis of bile salt resistance in Enterococcus faecium

    Primer                 Sequence a
delete_XmaI_gltK_up_F 5′-CCCCCCGGGCCAAGCAGGTACGATTGGAT-3′
delete_EcoRI_gltK_up_R 5′-AACCGGAAAGCAGAGAATTCTCGAAAACAATGAAACTTCAACA-3′
delete_EcoRI_gltK_dn_F 5′-TCGAGAATTCTCTGCTTTCCGGTTACTTGG-3′
delete_XhoI_gltK_dn_R 5′-CCGCTCGAGGGAAGGATCACACCGATGAC-3′
check_gltK_up 5′-CGGAACGTTAATGGCAATCT-3′
check_gltK_dn 5′-CCGTACCAATCGTACCGATAA-3′
delete_XmaI_gspA_up_F 5′-CCCCCCGGGCCTCCTTTTTGGACTTTCTCG-3′
delete_EcoRI_gspA_up_R 5′-ACCACATTTAGCTGCAGAATTCGACGGCTTTCCGTTGTGTAG-3′
delete_EcoRI_gspA_dn_F 5′-CGAATTCTGCAGCTAAATGTGGTACGAA-3′
delete_XhoI_gspA_dn_R 5′-CCGCTCGAGGCCAAGTGAAAGCTTTGGAA-3′
check_gspA_up 5′-GCTCGAATTCTTCGATTGCT-3′
check_gspA_dn 5′-TGATGAGCCGTTAAATGGAA-3′
complement_BamHI_gltK_F 5′-ACGGGATCCTTTTAGCAATCGTAGCTGGTTT-3′
complement_XhoI_gltK_R 5′-ACCGCTCGAGCGTGAATTTTCAAGTGCTC-3′
complement_BamHI_gspA_F 5′-ACGGGATCCTGAAAAACCTTCGATCGTTCA-3′
complement_XhoI_gspA_R 5′-ACCGCTCGAGTCCATTCCTACTCCCCCTCT-3′
pAT392_EcoRI_lox66_genta_F 5′-AGGGAATTCTACCGTTCGTATAGCATACATTATACGAAGTTATG ATAAACCCAGCGAACCATTTGAGG-3′
pAT392_EcoRI_lox71_genta_R 5′-CTCCGAATTCTACCGTTCGTATAATGTATGCTATACGAAGTTATT CAATCTTTATAAGTCCTTTTATAA-3′
  1. a Restriction sites are underlined.