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Table 1 Changes in rat brain cyp and arachidonate-related gene expression after traumatic brain injury

From: Temporal changes of cytochrome P450 (Cyp) and eicosanoid-related gene expression in the rat brain after traumatic brain injury

  HIPPOCAMPUS, time postinjury: CORTEX, time postinjury:
Gene name 6 h 24 h 72 h 168 h 6 h 24 h 72 h 168 h
  A. Arachidonate-related Cyp s: mRNA fold-changes*
Cyp2c6 b 3.5x    b b b b
Cyp2c22   3x       
Cyp2c23   3x       
Cyp2c54 b 3x b b b b b b
Cyp2e1   2.5x       
Cyp2j3     2x    2x [1.5x]
Cyp2j4    [2x] 4x    2x [1.5x]
Cyp2u1    1.5x 2.5x     
Cyp4a1   3x       
Cyp4a8 2x 4x 7x 2x     
Cyp4b1 [3x] 6x 5x 9x 2x 3x 4x [1.5x]
Cyp4f5    1.5x 2x    [1.5x] [1.5x]
Cyp4f6    1.5x 2x     
Cyp4f18      3x [2x] [1.5x]  
Cyp4x1      ½x ½x [½x] ½x
Cyp26b1     4x     2.5x
  B. Eicosanoid-related genes: mRNA fold-changes*
Alox15      4x [2x]   
Ptgds2    6x 8x    5x 4x
Ptges [2x] [3x] [3x] 11x 14.5x [4.5x] [1.5x] [1.5x]
Ptgis    [1.5x] 3.5x 2x 1.5x [2.5x] [2.5x]
Ptgs1    6x [½x]    2.5x [½x]
Ptgs2 3x   [½x] [½x] 5x [3x]   
Tbxas1    3x 5x [3.5x] [3x] 6x [3.5x]
Ephx1     2x     
Hpgd   1.5x       
Lta4h      [1.5x]   2.5x [1.5x]
Pla2g4a [2x] [2x] 3.5x 2.5x 2.5x 2.5x 5x [2x]
Alox5ap [2x] [3x] 4x 4x 5x [3x] [3x]  
Ptgfrn 1.5x 1.5x 1.5x 1.5x     
Ptgdr_pred    [1.5x] 3x    [2.5x] [2.5x]
Ptger2 2x [1.5x] 3x [2x] [2x] [2.5x] [2.5x]  
Ptger4 [2.5x] [2.5x] 5x 7x 2.5 1.5x 2.5x 1.5x
Ptgir 6x 5x 3x [2.5x] 4x 2.5x [2x]  
  C. Other Cyp genes: mRNA fold-changes*
Cyp1a1   2.5x 1.5x 1.5x     
Cyp1b1    3x 20x 5x 6.5x 3.5x  
Cyp2a2 b [~10x]      b  
Cyp2r1    [1.5x] 2.5x     
Cyp3a18   3x       
Cyp7a1 b 4x 6.5x 8x   b b  
Cyp11b3 9.5x 8.5x 2.5x      
Cyp17a1    3x      
Cyp20a1   [2x] [2x] 2.5x     
Cyp24a1      ~5.5x ~7x b  
Cyp27a1 [½x]   [3x] 9x   [½x] [1.5x] [2x]
Cyp27b1     2xª ~8x ~11x b [~2x]
  1. A: Arachidonate-related Cyps; B: Eicosanoid-related genes; C: Other Cyp genes. Gene expression assessed by qPCR is expressed as fold-change “x” compared to sham-operated controls at the same time point (i.e., 2-ΔΔCt). Only changes greater than 1.5x (or less than ½x) are shown. *p < 0.05, 2-way ANOVA, Tukey HSD (except where noted). Analyses performed on 2-ΔCt values of injured vs. sham at designated times after TBI (shown as fold-change rounded to the nearest ½x). ¶ p < 0.05, 1-way ANOVA with all shams combined as time zero. [x] brackets indicate changes ≥1.5-fold that were not statistically significant, but showed a trend (0.05 < p < 0.10). b Below the limit of quantification at these times. ~ Control (naïve, sham) values at or below limit of quantification, thus fold-change estimated based on highest control value(s).