Temporal expression of “hatching” enzymes. Gene expression levels (log2), from 1 to 33 dph, of S. solea transcripts codifying hatching enzymes. A. S. solea hatching enzymes (HCE and LCE); B. S. solea homologs of astacin-like protein; C. S. solea N_isotig00480. Graphics show mean gene expression measured with microarray, bars indicate standard deviation (SD) across biological replicates. Statistical significance (p < 0.05) when comparing one larval stage against the previous one is indicated by asterisk (*).