Table 3 List of primers used for PCR amplification of DYZ1 array (A) and SRY gene (B)
From: Fate of the human Y chromosome linked genes and loci in prostate cancer cell lines DU145 and LNCaP
(A) | |||||
|---|---|---|---|---|---|
Serial No. | Primer ID | Primer Sequence | Length (bp) | Location (DYZ1 array) | Orientation |
1 | SAS 1 | CCATTCGAGACCGTAGCAAT | 20 | 35-16 (5’ upstream to HaeIII site) | 5’-3’ |
2 | SAS 2 | TTTCCTTTCGCTTGCATTCCAT | 22 | 63-84 | 5’-3’ |
3 | SAS 3 | ATTTGATGCCATCCCATGAC | 20 | 763-782 | 5’-3’ |
4 | SAS 4 | TTTTGAGTCCGTTCCATAACAC | 22 | 1380-1401 | 5’-3’ |
5 | SAS 5 | TCCTTTGCCTTCCATTCG | 18 | 1668-1685 | 5’-3’ |
6 | SAS 6 | TGCAGTCTTTTCCCTTCGAG | 20 | 2564-2583 | 5’-3’ |
7 | SAS 7 | ATTGGATGGGATTGGAATGA | 20 | 861-880 | 3’-5’ |
8 | SAS 8 | TGGATGGACTGCAATAGAAAG | 22 | 1600-1621 | 3’-5’ |
9 | SAS 9 | TCGAATGGAAGGCAAAGG | 18 | 1669-1686 | 3’-5’ |
10 | SAS 10 | CGACTGGTACGGACTCCAAT | 20 | 2637-2656 | 3’-5’ |
11 | SAS 11 | GACTGGAAAGGCTGGGTGTCGA | 22 | 3419-3440 | 3’-5’ |
12 | SAS 12 | TGGACAGCCTGGAATAAAGTG | 21 | 3586-3606 | 3’-5’ |
(B) | |||||
1 | SA 531 | GAATCTGGTAGAAGTGAGTTTTGGA | 25 | 61-85 | 5’-3’ |
2 | SA 532 | GCCTTTATTAGCCAGAGAAAAGAAA | 25 | 860-884 | 3’-5’ |