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Table 4 List of DYZ 1 primer combinations used for end point PCR, their corresponding amplicon sizes and reaction conditions

From: Fate of the human Y chromosome linked genes and loci in prostate cancer cell lines DU145 and LNCaP

Serial No. DYZ1, Primer Combinations Amplicon Size (bp) PCR conditions (Annealing and Extension)
1 SAS (1&7) 915 64°C-1.0’, 72°C −1.0’
2 SAS (1&8) 1656 65°C −1.0’, 72°C −2.0’
3 SAS (1&9) 1721 61°C −1.0’, 72°C −2.0’
4 SAS (2&7) 818 61°C −1.0’, 72°C −1.0’
5 SAS (2&8) 1559 60-°C 1.0’, 72°C −1.5’
6 SAS (3&7) 118 55-°C 1.0’, 72°C −1.0’
7 SAS (3&8) 859 65°C −1.0’, 72°C −1.0’
8 SAS (3&9) 924 61°C −1.0’, 72°C −1.0’
9 SAS (3&10) 1894 64-°C 1.0’, 72°C −2.0’
10 SAS (4&8) 242 61°C −1.0’, 72°C −1.0’
11 SAS (4&9) 307 55°C −1.0’, 72°C −1.0’
12 SAS (4&10) 1277 61°C −1.0’, 72°C −1.5’
13 SAS (4&11) 2061 62°C −1.0’, 72°C −2.5’
14 SAS (4&12) 2192 61°C −1.0’, 72°C −2.5’
15 SAS (5&10) 989 61°C −1.0’, 72°C −1.0’
16 SAS (5&12) 1904 61°C −1.0’, 72°C −2.0’
17 SAS (6&10) 93 61°C −1.0’, 72°C −1.0’
18 SAS (6&11) 877 61°C −1.0’, 72°C −1.0’
19 SAS (6&12) 1008 64°C −1.0’, 72°C −1.0’