Table 6 Restriction mapping of 3.56 Kb DYZ1 sequence from DU145, LNCaP and the reference sequence AC068123.5 by virtual digest using Restriction Mapper Version 3 software
From: Fate of the human Y chromosome linked genes and loci in prostate cancer cell lines DU145 and LNCaP
Serial No. | Restriction enzyme | Sequence | Sight Length | Overhung | Frequency of cut sites | ||
|---|---|---|---|---|---|---|---|
AC068123.5 | DU145 | LNCaP | |||||
1 | BsaBI | GATNNNNATC | 6 | blunt | 1 | 0 | 0 |
2 | Cac8I | GCNNGC | 4 | blunt | 1 | 1 | 1 |
3 | NlaIV | GGNNCC | 4 | blunt | 1 | 0 | 0 |
4 | BamHI | GGATCC | 6 | 5’ | 1 | 0 | 0 |
5 | BseYI | CCCAGC | 6 | 5’ | 1 | 1 | 1 |
6 | Bsp1407I | TGTACA | 6 | 5’ | 1 | 1 | 1 |
7 | BspHI | TCATGA | 6 | 5’ | 1 | 1 | 1 |
8 | ClaI | ATCGAT | 6 | 5’ | 1 | 1 | 1 |
9 | DdeI | CTNAG | 4 | 5’ | 1 | 1 | 1 |
10 | EcoRI | GAATTC | 6 | 5’ | 1 | 1 | 1 |
11 | EcoRII | CCWGG | 5 | 5’ | 1 | 0 | 0 |
12 | MaeII | ACGT | 4 | 5’ | 1 | 1 | 0 |
13 | MaeIII | GTNAC | 4 | 5’ | 1 | 1 | 0 |
14 | VspI | ATTAAT | 6 | 5’ | 1 | 1 | 1 |
15 | XhoII | RGATCY | 6 | 5’ | 1 | 0 | 0 |
16 | BseMII | CTCAG | 5 | 3’ | 1 | 1 | 1 |
17 | BsgI | GTGCAG | 6 | 3’ | 1 | 1 | 1 |
18 | BtsI | GCAGTG | 6 | 3’ | 1 | 1 | 1 |
19 | Eco57I | CTGAAG | 6 | 3’ | 0 | 1 | 1 |
20 | Eco57MI | CTGRAG | 6 | 3’ | 1 | 2 | 2 |
21 | GsuI | CTGGAG | 6 | 3’ | 1 | 1 | 1 |
22 | Hpy188I | TCNGA | 4 | 3’ | 1 | 1 | 1 |
23 | MnlI | CCTC | 4 | 3’ | 1 | 1 | 1 |
24 | PflMI | CCANNNNNTGG | 6 | 3’ | 1 | 1 | 1 |
25 | SduI | GDGCHC | 6 | 3’ | 1 | 1 | 1 |
26 | ScrFI | CCNGG | 4 | 5’ | 2 | 1 | 1 |
27 | ArsI | GACNNNNNNTTYG | 7 | 3’ | 2 | 2 | 2 |
28 | BdaI | TGANNNNNNTCA | 6 | 3’ | 2 | 2 | 2 |
29 | CspCI | CAANNNNNGTGG | 7 | 3’ | 2 | 0 | 0 |
30 | TspRI | CASTG | 5 | 3’ | 2 | 2 | 2 |
31 | DpnI | GATC | 4 | blunt | 3 | 2 | 2 |
32 | ApoI | RAATTY | 6 | 5’ | 3 | 3 | 3 |
33 | AsuII | TTCGAA | 6 | 5’ | 3 | 3 | 3 |
34 | Eco31I | GGTCTC | 6 | 5’ | 3 | 2 | 2 |
35 | MaeI | CTAG | 4 | 5’ | 3 | 3 | 4 |
36 | MboI | GATC | 4 | 5’ | 3 | 2 | 2 |
37 | SfaNI | GCATC | 5 | 5’ | 3 | 4 | 4 |
38 | TatI | WGTACW | 6 | 5’ | 3 | 3 | 3 |
39 | SetI | ASST | 4 | 3’ | 3 | 3 | 1 |
40 | CviJI | RGCY | 4 | blunt | 4 | 4 | 4 |
41 | MslI | CAYNNNNRTG | 6 | blunt | 4 | 4 | 4 |
42 | FokI | GGATG | 5 | 5’ | 4 | 2 | 2 |
43 | AlfI | GCANNNNNNTGC | 6 | 3’ | 4 | 4 | 4 |
44 | RsaI | GTAC | 4 | blunt | 5 | 5 | 4 |
45 | BccI | CCATC | 5 | 5’ | 5 | 3 | 4 |
46 | BsmAI | GTCTC | 5 | 5’ | 5 | 5 | 4 |
47 | MseI | TTAA | 4 | 5’ | 5 | 6 | 6 |
48 | BstXI | CCANNNNNNTGG | 6 | 3’ | 5 | 5 | 4 |
49 | MboII | GAAGA | 5 | 3’ | 5 | 3 | 3 |
50 | XmnI | GAANNNNTTC | 6 | blunt | 6 | 6 | 6 |
51 | TstI | CACNNNNNNTCC | 6 | 3’ | 6 | 4 | 4 |
52 | NlaIII | CATG | 4 | 3’ | 7 | 7 | 7 |
53 | BcgI | CGANNNNNNTGC | 6 | 3’ | 16 | 14 | 14 |
54 | BsrDI | GCAATG | 6 | 3’ | 16 | 14 | 14 |
55 | BsrI | ACTGG | 5 | 3’ | 17 | 19 | 18 |
56 | TspGWI | ACGGA | 5 | 3’ | 20 | 23 | 17 |
57 | AgsI | TTSAA | 5 | 3’ | 22 | 20 | 23 |
58 | BsmI | GAATGC | 6 | 3’ | 22 | 23 | 21 |
59 | FaiI | YATR | 4 | blunt | 23 | 25 | 23 |
60 | PleI | GAGTC | 5 | 5’ | 23 | 21 | 21 |
61 | TspDTI | ATGAA | 5 | 3’ | 25 | 24 | 24 |
62 | TspEI | AATT | 4 | 5’ | 35 | 35 | 35 |
63 | TfiI | GAWTC | 5 | 5’ | 52 | 51 | 51 |
64 | TaqI | TCGA | 4 | 5’ | 66 | 62 | 62 |
65 | HinfI | GANTC | 4 | 5’ | 75 | 72 | 72 |