Figure 6

Association of CREB binding sites with fasting-responsive TATA-box containing promoters. CREB sites were separated into 3 groups: 285 sites associated with genes up-regulated in fasted state (red), 321 sites associated with genes down-regulated in fasted state (green), and 6,018 sites associated with genes that did not change (gray). A) Rates of CRE motif sequences (either full CRE or half CRE) identified for each group of CREB sites. *p-value < 0.05 by Fisher’s exact test versus the ‘No Change’ group. B) Rates of CREB sites, separated by association with fasting-dependent regulation, occurring in proximal promoter (−2 kb to +200 bp around TSS), distal promoter (−10 kb to -2 kb upstream of TSS), intronic, and exonic regions. *p-value < 0.001 by Fisher’s exact test compared to corresponding gray bar. C) All genes on our microarray were separated into groups based on associated CREB binding and presence of a TATA-box in the promoter. For each group, the percentage of genes induced (red) and repressed (green) in response to fasting is shown. *p-value < 1E-11, by Fisher’s Exact Test against the CREB-/TATA- group. NS indicates p-value > 0.05.