Figure 3

Reverse transcriptase real-time PCR verification of expression changes in canine miRNAs miR-200c, miR-31, miR-196a, miR-21, miR-382 and miR-885 (panels A - F respectively). Data are mean relative expression units, controlled for reverse transcription efficiency and normalised to miR-191, in cervical subcutaneous tissue following administration of vehicle (control) or MMPi for 4, 8, 11, 14 and 17 days + S.E.M (n = 5). Data are analysed by one-way analysis of variance with Dunnett's post hoc test (comparing all experimental groups to vehicle) where P < 0.05; * denotes P < 0.05, ** denotes P < 0.01 and *** denotes P < 0.001.