Figure 4

The top two scoring gene-networks (A and C) of dysregulated miRNAs and mRNAs, and associated biological functions (B and D) in response to 4 days administration of MMPi as determined by Ingenuity Pathway Analysis.4A and 4C - Data are mean fold change represented graphically - green colouration denotes significant down-regulation whilst red colouration denotes significant up regulation (P < 0.05). Arrows between molecules denote a stimulatory effect and blocked arrows denote a suppressive effect. Note: Transcription factors Sp1, RelA, and STAT1 are included due to their association with gene changes involved in the development of MMPi induced fibrodysplasia. Network score = −log (Fishers Exact Test result); Molecule Count refers to the number of molecules included within the network for which expression data is available (miRNA or mRNA). 4B and 4D - Data are calculated P values (−Log) determined by the Fishers Exact Test for the most significant biological functions associated with the top two scoring gene networks. Significance was accepted were P < 0.05 (−Log P > 1.301) as shown by the threshold line in orange.