Figure 1
From: Identification of thyroid hormone receptor binding sites in developing mouse cerebellum
Microarray signal intensity biases and post normalization plots with respect to nucleotide composition and count. Both biases follow a polynomial trend that is unrelated to ChIP-chip signal. Since there is no relation between probe characteristics and signal, one should expect no trend in the data. a. The effect of base position along the probe on the signal intensity for the TI channel. A quadratic effect was observed for base position along the probe for A, C and G. b. Median log2 signal intensity of TI probes on the microarray versus the number of individual nucleotides in each probe.