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Table 3 qRT-PCR validation of representative genes that are differentially expressed between control and PCV2-infected PAMs

From: Transcription analysis of the porcine alveolar macrophage response to porcine circovirus type 2

Gene GenBank ID 24 HPI 48 HPI
   Microarray FC qRT-PCR FC Microarray FC qRT-PCR FC
CD14 NM_001097445.2 3.60 2.35(0.041) 3.87 2.71(0.003)
GPNMB NM_001098584 −2.13 −4.04(<0.0001) −2.58 −7.89(0.002)
ICAM1 NM_000201.2 4.02 3.59(<0.0001) 3.20 2.21(0.003)
IFNγ NM_213948.1 −2.04 −2.76(0.005) 3.18 5.09(0.006)
IL6 NM_214399.1 2.26 4.60(0.001) 1.36 2.47(0.001)
IL8 NM_213867.1 4.86 8.65(0.001) 5.03 5.36(0.008)
ISG15 EU584557.1 1.56 1.37(0.006) 2.51 2.83(0.031)
S100A1 NM_006271 −3.20 −4.42 (0.001) −2.49 −1.87 (0.01)
S100A12 NM_001160272.1 10.98 8.49(0.006) 5.13 3.98(0.005)
S100A4 XM_001929560 −3.14 −4.26(0.002) −1.98 −3.27(0.001)
S100A8 FJ263391.1 12.96 15.47(<0.0001) 6.83 4.34(0.003)
S100A9 NM_001177906.1 20.00 16.56(0.007) 9.71 6.21(0.025)
TLR1 NM_001031775.1 2.76 2.29 (0.032) 5.83 4.89(0.016)
TLR8 NM_214187.1 −4.35 −3.01(0.002) −1.14 −1.72(0.004)
TLR9 AY859728.1 2.47 4.66(0.012) 1.43 2.24(0.0001)
TNFα EU682384.1 5.13 5.36(0.016) 1.96 2.53(0.022)
VCAM1 NM_213891 −2.33 −7.54(0.001) −2.90 −4.83 (0.005)
  1. All reactions were performed in triplicate. FC: fold-change.
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