Skip to main content

Table 3 qRT-PCR validation of representative genes that are differentially expressed between control and PCV2-infected PAMs

From: Transcription analysis of the porcine alveolar macrophage response to porcine circovirus type 2

Gene

GenBank ID

24 HPI

48 HPI

  

Microarray FC

qRT-PCR FC

Microarray FC

qRT-PCR FC

CD14

NM_001097445.2

3.60

2.35(0.041)

3.87

2.71(0.003)

GPNMB

NM_001098584

−2.13

−4.04(<0.0001)

−2.58

−7.89(0.002)

ICAM1

NM_000201.2

4.02

3.59(<0.0001)

3.20

2.21(0.003)

IFNγ

NM_213948.1

−2.04

−2.76(0.005)

3.18

5.09(0.006)

IL6

NM_214399.1

2.26

4.60(0.001)

1.36

2.47(0.001)

IL8

NM_213867.1

4.86

8.65(0.001)

5.03

5.36(0.008)

ISG15

EU584557.1

1.56

1.37(0.006)

2.51

2.83(0.031)

S100A1

NM_006271

−3.20

−4.42 (0.001)

−2.49

−1.87 (0.01)

S100A12

NM_001160272.1

10.98

8.49(0.006)

5.13

3.98(0.005)

S100A4

XM_001929560

−3.14

−4.26(0.002)

−1.98

−3.27(0.001)

S100A8

FJ263391.1

12.96

15.47(<0.0001)

6.83

4.34(0.003)

S100A9

NM_001177906.1

20.00

16.56(0.007)

9.71

6.21(0.025)

TLR1

NM_001031775.1

2.76

2.29 (0.032)

5.83

4.89(0.016)

TLR8

NM_214187.1

−4.35

−3.01(0.002)

−1.14

−1.72(0.004)

TLR9

AY859728.1

2.47

4.66(0.012)

1.43

2.24(0.0001)

TNFα

EU682384.1

5.13

5.36(0.016)

1.96

2.53(0.022)

VCAM1

NM_213891

−2.33

−7.54(0.001)

−2.90

−4.83 (0.005)

  1. All reactions were performed in triplicate. FC: fold-change.