Table 1 Alignment result of the HLA gene sequence and genotype in the HLA homozygous cell lines
From: Phase-defined complete sequencing of the HLA genes by next-generation sequencing
Cell line | Gene | Allele | Average depth | False heterozygous SNVs | Coverage* | Closest HLA genotype | Note |
|---|---|---|---|---|---|---|---|
AKIBA | A | 24:02 | 219.5 | 0 | 100 | A*24:02:01:01 | Â |
| Â | C | 12:02:02 | 655.5 | 0 | 100 | C*12:02:02 | Â |
| Â | B | 52:01 | 74.0 | 0 | 100 | B*52:01:01:02 | Â |
| Â | DRB1 | 15:02 | 346.9 | 0 | 100 | DRB1*15:02:01 | Â |
| Â | DQB1 | 06:01:01 | 33.2 | 0 | 100 | DQB1*06:01:01 | Â |
| Â | DPB1 | 09:01 | 604.9 | 0 | 100 | DPB1*09:01 | Â |
AMAI | A | 68:02 | 35.2 | 0 | 100 | A*68:02:01:01 | Â |
| Â | C | 04:01 | 159.3 | 0 | 100 | C*04:01:01:01 | Â |
| Â | B | 53:01 | 52.2 | 0 | 100 | B*53:01:01 | Â |
| Â | DRB1 | 15:03 | 92.6 | 0 | 100 | DRB1*15:03:01 | 2bp (TG) deletion |
| Â | DQB1 | 06:02 | 133.6 | 0 | 100 | DQB1*06:02:01 | Â |
| Â | DPB1 | 04:02 | 94.9 | 0 | 100 | DPB1*105:01 | different from ECACC |
ARBO | A | 03:01 | 133.5 | 0 | 100 | A*03:01:01:01 | Â |
| Â | C | 06:02 | 208.8 | 0 | 100 | C*06:02:02:01 | Â |
| Â | B | 58:02 | 108.2 | 0 | 100 | B*58:02 | Â |
| Â | DRB1 | 15:03 | 43.4 | - | 86.6 | not determined | Â |
| Â | DQB1 | 06:02 | 40.0 | - | 71.5 | not determined | Â |
| Â | DPB1 | 04:02 | 100.6 | 0 | 99.2 | not determined | Â |
BM15 | A | 01:01 | 79.3 | 0 | 100 | A*01:01:01:01 | Â |
| Â | C | 07:01 | 198.7 | 0 | 100 | C*07:01:01 | Â |
| Â | B | 49:01 | 34.0 | 0 | 100 | B*49:01:01 | Â |
| Â | DRB1 | 11:02 | 90.9 | 0 | 98.3 | not determined | Â |
| Â | DQB1 | 03:01 | 14.2 | 0 | 100 | DQB1*03:01:01:01 | 2 intronic and 1 nonsynonymous novel variants |
| Â | DPB1 | 03:01 | 132.0 | 0 | 100 | DPB1*03:01:01 | 24 intronic novel variants |
BM16 | A | 02:01 | 28.6 | 0 | 100 | A*02:01:01:01 | Â |
| Â | C | 07:01 | 249.0 | 0 | 100 | C*07:01:01:01 | Â |
| Â | B | 18:01 | 183.3 | 0 | 100 | B*18:01:01 | 3 intronic novel variants |
| Â | DRB1 | 12:01 | 55.4 | 0 | 100 | DRB1*12:01:01 | 43 intronic novel variants |
| Â | DQB1 | 03:01 | 36.3 | 0 | 100 | DQB1*03:01:01:01 | 1 intronic novel variant |
| Â | DPB1 | 02:01:02 | 58.3 | 0 | 100 | DPB1*02:01:02 | 4bp (GGAA) insertion |
BM21 | A | 01:01 | 61.0 | 0 | 100 | A*01:01:01:01 | Â |
| Â | C | 17:01 | 233.0 | 0 | 100 | C*17:01:01:02 | Â |
| Â | B | 41:01 | 176.3 | 0 | 100 | B*41:01 | Â |
| Â | DRB1 | 11:01 | 80.0 | 0 | 100 | DRB1*11:01:01 | Â |
|  | DQB1 | 03:01 | 17.8 | 9†| 99.7 | DQB1*03:01:01:01 |  |
| Â | DPB1 | 10:01 | 103.8 | 0 | 100 | DPB1*10:01 | Â |
BM92 | A | 25:01:01 | 24.1 | 0 | 100 | A*25:01:01 | Â |
| Â | C | 01:02 | 458.0 | 0 | 100 | C*01:02:01 | Â |
| Â | B | 51:01:01 | 218.0 | 0 | 100 | B*51:01:01 | Â |
| Â | DRB1 | 04:04 | 2.9 | - | 32.9 | not determined | Â |
| Â | DQB1 | 03:01 | 1.1 | - | 0 | not determined | Â |
| Â | DPB1 | 04:02 | 115.6 | 0 | 100 | DPB1*04:02:01 | Â |
Boleth | A | 02:01:01:01 | 25.7 | 1†| 100 | A*02:01:01:01 |  |
| Â | C | 03:04 | 273.3 | 0 | 100 | C*03:04:01:01 | Â |
| Â | B | 15:01 | 260.2 | 0 | 100 | B*15:01:01:01 | Â |
| Â | DRB1 | 04:01:01 | 27.1 | 0 | 98.4 | not determined | Â |
| Â | DQB1 | 03:02:01 | 67.2 | 0 | 100 | DQB1*03:02:01 | Â |
| Â | DPB1 | 04:01:01 | 99.1 | 0 | 100 | DPB1*04:01:01 | Â |
BSM | A | 02:01 | 56.9 | 0 | 100 | A*02:01:01:01 | Â |
| Â | C | 03:04 | 238.6 | 0 | 100 | C*03:04:01:01 | Â |
| Â | B | 15:01 | 204.3 | 0 | 100 | B*15:01:01:01 | Â |
| Â | DRB1 | 04:01 | 18.3 | 0 | 98.4 | not determined | Â |
| Â | DQB1 | 03:02 | 91.1 | 0 | 100 | DQB1*03:02:01 | Â |
| Â | DPB1 | 02:01:02 | 57.6 | 0 | 100 | DPB1*02:01:02 | Â |
Carogero | A | 02:01 | 28.2 | 0 | 100 | A*02:01:01:01 | Â |
| Â | C | 20:22 | 22.8 | 0 | 100 | C*02:02:02 | Â |
| Â | B | 40:02 | 6.1 | - | 91 | not determined | Â |
| Â | DRB1 | - | 16.8 | 0 | 100 | DRB1*16:01:02 | Â |
| Â | DQB1 | - | 27.5 | 0 | 100 | DQB1*05:02:01 | Â |
| Â | DPB1 | 04:01 | 18.8 | 0 | 99.7 | not determined | Â |
COX | A | 01:01:01:01 | 99.1 | 0 | 100 | A*01:01:01:01 | Â |
| Â | C | 07:01:01 | 322.2 | 0 | 100 | C*07:01:01 | Â |
| Â | B | 08:01:01 | 123.5 | 0 | 100 | B*08:01:01 | Â |
| Â | DRB1 | 03:01 | 284.9 | 0 | 100 | DRB1*03:01:01 | Â |
| Â | DQB1 | 02:01 | 367.3 | 0 | 100 | DQB1*02:01:01 | Â |
| Â | DPB1 | 03:01 | 270.5 | 0 | 100 | DPB1*03:01:01 | Â |
DBB | A | 02:01 | 454.2 | 0 | 100 | A*02:01:01 | Â |
| Â | C | 06:02 | 636.8 | 0 | 100 | C*06:02:01:01 | Â |
| Â | B | 57:01 | 142.5 | 0 | 100 | B*57:01:01 | Â |
| Â | DRB1 | 07:01 | 14.3 | - | 90.7 | not determined | Â |
| Â | DQB1 | 30:32 | 1.9 | - | 70.8 | not determined | Â |
| Â | DPB1 | 04:01 | 568.0 | 0 | 100 | DPB1*04:01:01:01 | Â |
DHI | A | 31:01 | 2.2 | - | 12.9 | not determined | Â |
| Â | C | 12:03 | 292.9 | 0 | 100 | C*12:03:01:01 | Â |
| Â | B | 38:01 | 250.1 | 0 | 100 | B*38:01:01 | Â |
| Â | DRB1 | 11:01 | 57.2 | 0 | 100 | DRB1*11:01:01 | Â |
| Â | DQB1 | 03:01 | 140.1 | 0 | 100 | DQB1*03:01:01:03 | Â |
| Â | DPB1 | 04:01 | 302.2 | 0 | 100 | DPB1*04:01:01:01 | Â |
DKB | A | 24:02 | 358.6 | 0 | 100 | A*24:02:01 | 1 intronic novel variant |
| Â | C | 03:04 | 502.8 | 0 | 100 | C*03:04:01:01 | Â |
| Â | B | 40:01 | 634.1 | 0 | 100 | B*40:01:02 | Â |
| Â | DRB1 | 90:12 | 61.4 | - | 65 | not determined | Â |
| Â | DQB1 | 30:32 | 310.1 | 0 | 100 | DQB1*03:03:02:02 | Â |
| Â | DPB1 | 04:01 | 259.2 | 0 | 100 | DPB1*04:01:01:01 | Â |
HARA | A | 24:02:01:01 | 122.6 | 0 | 100 | A*30:01:01 | different from ECACC |
| Â | C | 12:02 | 214.3 | 0 | 100 | C*12:02:02 | Â |
| Â | B | 52:01:01 | 189.1 | 0 | 100 | B*52:01:01:02 | Â |
| Â | DRB1 | 15:02:01 | 4.5 | 0 | 97.5 | not determined | Â |
| Â | DQB1 | 06:01 | 124.6 | 0 | 100 | DQB1*06:01:01 | Â |
| Â | DPB1 | 09:01 | 52.9 | - | 97.6 | not determined | Â |
HHK | A | 03:01 | 265.9 | 0 | 100 | A*03:01:01:01 | Â |
| Â | C | 07:02 | 84.6 | 0 | 100 | C*07:02:01:03 | Â |
| Â | B | 07:02 | 11.5 | 0 | 100 | B*07:02:01 | Â |
| Â | DRB1 | 13:01 | 47.7 | 0 | 100 | DRB1*13:01:01 | Â |
| Â | DQB1 | 06:03 | 103.3 | 0 | 100 | DQB1*06:03:01 | Â |
| Â | DPB1 | 04:01 | 65.1 | 0 | 100 | DPB1*04:01:01:01 | Â |
HOKKAIDO | A | - | 8.0 | 0 | 100 | A*24:02:01:01 | Â |
| Â | C | - | 42.8 | 0 | 100 | C*03:04:01:02 | Â |
| Â | B | - | 99.0 | 0 | 100 | B*54:01:01 | Â |
| Â | DRB1 | 04:05 | 8.2 | - | 11.6 | not determined | Â |
| Â | DQB1 | 04:01 | 48.4 | - | 57.4 | not determined | Â |
| Â | DPB1 | 05:01 | 70.4 | 0 | 100 | DPB1*05:01:01 | Â |
JBUSH | A | 32:01 | 10.9 | 1†| 100 | A*32:01:01 |  |
| Â | C | 12:03 | 128.8 | 0 | 100 | C*12:03:01:01 | Â |
| Â | B | 38:01 | 298.3 | 0 | 100 | B*38:01:01 | Â |
| Â | DRB1 | 11:01 | 23.7 | 0 | 100 | DRB1*11:01:03 | Â |
| Â | DQB1 | 03:01 | 80.1 | 0 | 100 | DQB1*03:01:01 | Â |
| Â | DPB1 | 04:01 | 86.7 | 0 | 100 | DPB1*04:01:01:01 | Â |
JEST | A | 02:01 | 11.3 | 0 | 100 | A*02:01:01 | Â |
| Â | C | 01:02 | 80.3 | 0 | 100 | C*01:02:01 | Â |
| Â | B | 27:05:02 | 76.8 | 0 | 100 | B*27:05:02 | 1 intronic novel variant |
| Â | DRB1 | 01:01 | 16.3 | 0 | 100 | DRB1*01:01:01 | Â |
| Â | DQB1 | 05:01 | 87.5 | 0 | 100 | DQB1*05:01:01 | 1 intronic novel variant |
| Â | DPB1 | 04:01 | 69.1 | 0 | 100 | DPB1*04:01:01:01 | Â |
K265 | A | - | 127.1 | 0 | 100 | A*03:01:01:01 | Â |
| Â | C | 12:02 | 163.7 | 0 | 100 | C*07:02:01:03 | Â |
| Â | B | - | 54.3 | 0 | 100 | B*07:02:01 | Â |
| Â | DRB1 | 15:02 | 92.7 | 0 | 100 | DRB1*15:01:01 | Â |
| Â | DQB1 | - | 257.5 | 0 | 100 | DQB1*06:02:01 | Â |
| Â | DPB1 | - | 107.8 | 0 | 100 | DPB1*04:01:01:01 | Â |
LBUF | A | 30:01:01 | 26.9 | 0 | 100 | A*30:01:01 | Â |
| Â | C | 06:02 | 326.3 | 0 | 100 | C*06:02:01:01 | Â |
| Â | B | 13:02:01 | 249.1 | 0 | 100 | B*13:02:01 | Â |
| Â | DRB1 | 07:01:01 | 16.6 | 0 | 100 | DRB1*07:01:02 | Â |
| Â | DQB1 | 02:02 | 2.9 | - | 18.9 | not determined | Â |
| Â | DPB1 | 17:01 | 177.9 | 0 | 100 | DPB1*17:01 | Â |
LKT3 | A | 24:02 | 86.9 | 0 | 100 | A*24:02:01 | Â |
| Â | C | 01:02 | 213.3 | 0 | 100 | C*01:02:01 | Â |
| Â | B | 54:01 | 48.7 | 0 | 100 | B*54:01:01 | Â |
| Â | DRB1 | 08:01 | 23.4 | - | 55.4 | not determined | Â |
| Â | DQB1 | 04:01 | 1.1 | - | 0 | not determined | Â |
| Â | DPB1 | 05:01 | 227.8 | 0 | 100 | DPB1*05:01:01 | Â |
MADULA | A | 02:01 | 86.8 | 0 | 100 | A*02:01:01 | Â |
| Â | C | 03:04 | 206.9 | 0 | 100 | C*03:04:01 | Â |
| Â | B | 40:01 | 223.8 | 0 | 100 | B*40:01:02 | Â |
| Â | DRB1 | 08:01 | 42.6 | 0 | 100 | DRB1*08:01:03 | Â |
| Â | DQB1 | 04:02 | 123.9 | - | 86.3 | not determined | Â |
| Â | DPB1 | 04:01 | 215.6 | 0 | 100 | DPB1*04:01:01 | Â |
PITOUT | A | 29:02 | 91.5 | 0 | 100 | A*29:02:01 | Â |
| Â | C | 16:01 | 51.8 | 0 | 100 | C*16:01:01 | Â |
| Â | B | 44:03:01 | 74.6 | 0 | 99.4 | not determined | Â |
| Â | DRB1 | 07:01 | 3.2 | Â | 40.4 | not determined | Â |
| Â | DQB1 | 02:01 | 38.3 | 0 | 100 | DQB1*02:02 | different from ECACC |
| Â | DPB1 | 04:01 | 340.7 | 0 | 100 | DPB1*04:01:01 | Â |
RMAL | A | 02:04 | 75.8 | 0 | 100 | A*02:04 | Â |
| Â | C | 15:02 | 68.6 | 0 | 100 | C*15:02:01 | Â |
| Â | B | 51:01 | 21.9 | 0 | 100 | B*51:01:01 | Â |
| Â | DRB1 | 16:02 | 63.5 | 0 | 100 | DRB1*16:02:02 | 3 intronic novel variants |
| Â | DQB1 | 03:01 | 16.2 | 0 | 100 | DQB1*03:01:01 | Â |
| Â | DPB1 | 04:02 | 66.8 | 0 | 100 | DPB1*04:02:01 | Â |
SAVC | A | 03:01 | 99.4 | 0 | 100 | A*03:01:01:01 | Â |
| Â | C | 07:02 | 140.3 | 0 | 100 | C*07:02:01 | Â |
| Â | B | 07:02 | 45.3 | 0 | 100 | B*07:02:01 | Â |
| Â | DRB1 | 04:01 | 2.4 | - | 50.3 | not determined | Â |
| Â | DQB1 | 03:02 | 1.2 | - | 12.9 | not determined | Â |
| Â | DPB1 | 10:01 | 129.2 | 0 | 100 | DPB1*10:01 | Â |
SRACH | A | 31:01 | 14.5 | - | 1.5 | not determined | Â |
| Â | C | 01:02 | 388.3 | 0 | 100 | C*01:02:01 | Â |
| Â | B | 15:01 | 688.5 | 0 | 100 | B*15:01:0101 | Â |
| Â | DRB1 | 08:02:01 | 61.5 | 0 | 100 | DRB1*08:02:01 | Â |
| Â | DQB1 | 04:02 | 174.0 | - | 91 | not determined | Â |
| Â | DPB1 | 04:02 | 207.0 | 0 | 100 | DPB1*04:02:01 | Â |
T182 | A | 24:02 | 12.4 | 0 | 100 | A*24:02:01:01 | Â |
| Â | C | 12:02:02 | 113.5 | 0 | 100 | C*12:02:02 | Â |
| Â | B | 52:01 | 106.0 | 0 | 100 | B*52:01:01:02 | Â |
| Â | DRB1 | 15:02 | 4.9 | - | 0 | not determined | Â |
| Â | DQB1 | 06:01 | 72.2 | 0 | 100 | DQB1*06:01:01 | Â |
| Â | DPB1 | 09:01 | 69.2 | 0 | 98.3 | not determined | Â |
TAB089 | A | 02:07 | 13.3 | 0 | 98.3 | not determined | Â |
| Â | C | 01:02 | 101.9 | 0 | 100 | C*01:02:01 | Â |
| Â | B | 46:01 | 36.9 | 0 | 100 | B*46:01:01 | Â |
| Â | DRB1 | 02:02 | 2.7 | - | 91.1 | not determined | Â |
|  | DQB1 | 06:01 | 49.1 | 1†| 100 | DQB1*06:01:01 |  |
| Â | DPB1 | 02:02 | 39.2 | 0 | 100 | DPB1*02:02 | 9 intronic novel variants |
TOK | A | 24:02 | 286.2 | 0 | 100 | A*24:02:01:01 | Â |
| Â | C | 12:02:02 | 241.5 | 0 | 100 | C*12:02:02 | Â |
| Â | B | 52:01 | 127.9 | 0 | 100 | B*52:01:01:02 | Â |
| Â | DRB1 | 15:02 | 163.7 | 0 | 100 | DRB1*15:02:01 | Â |
| Â | DQB1 | 06:01 | 243.1 | 0 | 100 | DQB1*06:01:01 | Â |
| Â | DPB1 | 09:01 | 175.2 | 0 | 100 | DPB1*09:01 | Â |
VAVY | A | 01:01 | 36.1 | 0 | 100 | A*01:01:01:01 | Â |
| Â | C | 07:01 | 141.6 | 0 | 100 | C*07:01:01 | Â |
| Â | B | 08:01 | 535.1 | 0 | 100 | B*08:01:01 | Â |
| Â | DRB1 | 03:01 | 43.4 | 0 | 100 | DRB1*03:01:01 | Â |
| Â | DQB1 | 02:01 | 126.4 | 0 | 100 | DQB1*02:01:01:01 | Â |
| Â | DPB1 | 01:01 | 57.0 | 0 | 100 | DPB1*01:01:01 | Â |
WT100 | A | 11:01 | 66.4 | 0 | 100 | A*11:01:01 | Â |
| Â | C | 04:01 | 112.9 | 0 | 100 | C*04:01:01:02 | Â |
| Â | B | 35:01 | 15.9 | 0 | 100 | B*35:01:01 | Â |
| Â | DRB1 | 01:01 | 143.5 | 0 | 100 | DRB1*01:01:01 | Â |
| Â | DQB1 | 05:01 | 136.4 | 0 | 100 | DQB1*05:01:01 | 1 intronic novel variant |
| Â | DPB1 | 01:01 | 122.0 | 0 | 100 | DPB1*01:01:01 | Â |
WT47 | A | 32:01 | 56.5 | 0 | 100 | A*32:01:01 | Â |
| Â | C | 05:01 | 192.5 | 0 | 100 | C*05:01:01 | Â |
| Â | B | 44:02 | 47.8 | 0 | 100 | B*44:02:01 | Â |
| Â | DRB1 | 13:02 | 116.1 | 0 | 100 | DRB1*13:02:01 | 4 intronic novel variants |
| Â | DQB1 | 06:04 | 807.8 | 0 | 100 | DQB1*06:04:01 | Â |
| Â | DPB1 | 16:01 | 172.2 | 0 | 100 | DPB1*16:01 | Â |