Figure 6

Relative expression levels analysis of four circadian rhythms related transcipts ( ELF1 , ELF2 , SFR6 , SUS ) in the Embryo (Em□) and endosperm (En■) under warm (20°C)/cold (4°C) seed stratification process. RS, radical just sprouted; RG, radical growing up to 2 cm. Expression is shown relative to GAPDH as the reference gene. RNA was extracted from three biological samples and qRT-PCR was performed. Data represent means ± SD. * indicate a significant difference between Em and En at P < 0.05.