Genetic association of OPRgenes with resistance to Hessian fly in hexaploid wheat

BMC Genomics

Table 1 Primers used for detecting allelic variation in TaOPR1-A, TaOPR2-A, TaOPR7-B, TaLOX1-A, TaLOX2-B, TaSrk, TaClp , and TaPkc in hexaploid wheat

Primer	Sequence (5′-3′)	Loci	PCR size (bp)	T_m(°C)	Restriction site
OPRC1-ABD-F2	CCGTCGACGCCGGTACG	TaOPR1-A	337	62	Kpnl
OPRC1-R8	GGCCGCCGATCTCCCT	TaOPR1-A
OPR22-C1-F3	TCTGCTTTCCTCTGCTCGTC	TaOPR2-A	634	55	Hincll
OPR-R2	TTCATGGTTCAATGACACATCAAGG	TaOPR2-A
OP1-C1F1	AAAAGGTTTTCACCTCGATGATCGGGG	TaOPR7-B	487	55	Ddel
OP1-R1	CCGATGGCCCTGCACCTCGTCATCG	TaOPR7-B
LOX-C5-F5	CATCCTGAATAAAGAACCTC	TaLOX1-A	2500a	55	ScrFl
LOX-C5-R6	GATCATATGGAGACGCTGTT	TaLOX1-A
LOX0-F4	GGAGCACGGCCTCAAGCTC	TaLOX2-B	680a	52b	dominant band
LOX0-R6	CATGTGGTTTATTTTAGCTCTGTAGA	TaLOX2-B
LOX-F4	CTGCGTCGAGCCCTACATCATCG	TaLOX6-B	264/272	62	8-bp indels
LOX-R6	GGGCCAGCCCCCGGCTGACG	TaLOX6-B
Srk-F3	CGGACCACTTCAACCATAGG	TaSrk	1437	56
Srk-R2	ACCTTTCACAGTCTGCAATGGCAATGCT	TaSrk
CLP-F1	TCCATCTCCTATGGCTTCTT	TaClp	1648	55	Rsal
CLP-R2	GGTGGGGGTTGCCTATCCAG	TaClp
Pkc-F3	GAGAGAAGCAATTCAGGGCT	TaPkc	911	55
Pkc-R2	CCAGATTACAATTTTAAAGAGAAG	TaPkc

^a Estimated product size.
^b A touch-down program (60°C down to 52°C for annealing temperature) was performed before the regular was performed (see details in Methods).

ISSN: 1471-2164