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Table 1 Assembly statistics for k-mer lengths 19–49 and different k-mer merge ranges from the Oases de novo assembly program

From: RNA-seq analysis reveals extensive transcriptional plasticity to temperature stress in a freshwater fish species

  k19 k21 k23 k25 k27 k29 k31 k33 k35 k37 k39 k41 k43 k45 k47 k49 k19_39 k21_39 k25_47 k21_49
Total sequences 1.2E+05 7.3E+04 6.2E+04 5.5E+04 5.2E+04 5.0E+04 4.8E+04 7.0E+04 8.2E+04 8.1E+04 8.2E+04 4.3E+04 4.1E+04 4.0E+04 3.9E+04 5.6E+04 4.5E+05 1.1E+05 4.0E+05 4.9E+05
Total bases 6.7E+07 6.1E+07 5.6E+07 5.3E+07 5.1E+07 5.0E+07 4.9E+07 6.2E+07 7.2E+07 7.3E+07 7.2E+07 4.4E+07 4.3E+07 4.2E+07 4.1E+07 5.1E+07 4.8E+08 1.3E+08 4.4E+08 5.5E+08
Min sequence length 7.1E+01 1.0E+02 8.1E+01 1.0E+02 9.8E+01 1.0E+02 9.0E+01 1.0E+02 1.0E+02 1.0E+02 1.0E+02 9.9E+01 1.0E+02 1.0E+02 1.0E+02 1.0E+02 1.0E+02 1.0E+02 1.0E+02 1.0E+02
Max sequence length 1.5E+04 1.7E+04 2.0E+04 1.8E+04 2.1E+04 2.3E+04 1.8E+04 1.2E+04 1.3E+04 1.3E+04 1.4E+04 1.7E+04 1.7E+04 1.7E+04 1.7E+04 1.4E+04 2.3E+04 1.7E+04 2.3E+04 2.3E+04
Average sequence length 558.04 837.19 906.27 960.63 979.44 991.21 1010.47 884.15 888.33 901.83 884.62 1026.19 1037.21 1042.06 1049.7 903.57 1071.74 1245.3 1114.01 1124.62
Median sequence length 356 527 546 580 584 590 605 595 583 584 568 624 634 639 650 608 780 930 805 818
N50 length 873 1397 1585 1686 1746 1759 1801 1398 1460 1493 1492 1785 1789 1795 1786 1457 1589 1856 1671 1689
(A + T)s 55.25% 55.32% 55.25% 55.16% 55.16% 55.17% 55.12% 55.07% 55.21% 55.35% 55.38% 55.04% 55.03% 55.05% 55.03% 54.85% 55.95% 55.11% 55.69% 56.13%
(G + C)s 43.99% 44.27% 44.47% 44.60% 44.64% 44.67% 44.72% 44.83% 44.63% 44.49% 44.48% 44.86% 44.87% 44.87% 44.89% 45.08% 44.05% 44.89% 44.31% 43.87%
Ns 0.77% 0.41% 0.28% 0.23% 0.21% 0.16% 0.16% 0.10% 0.16% 0.16% 0.14% 0.10% 0.10% 0.08% 0.08% 0.06% 0.00% 0.00% 0.00% 0.00%