ATR-FTIR spectroscopy reveals genomic loci regulating the tissue response in high fat diet fed BXD recombinant inbred mouse strains

Table 3 QTLs for the macromolecular composition of different tissues in BXD RI strains

Tissue	Chr	Trait	LRS	Significance	Increasing allele	Additive Effect
Reproductive	1	total fat	13.61	*	B6	198.54
Adipose		(15084)	11.92	*	B6	188.92
Tissue	1	saturated fat	13.71	*	B6	57.78
		(15083)	12.07	*	B6	55.09
	1	unsaturated fat	13.4	*	B6	1.26
		(15082)	11.76	*	B6	1.20
	1	acyl chain length	13.16	*	B6	0.36
		(15085)	10.82	*	B6	0.36
	1	collagen	13.24	*	B6	0.34
		(15089)	11.95	*	B6	0.33
	1	collagen integrity	10.62	*	D2	3.39
		(15090)	11.96	*	D2	3.80
			11.38	*	D2	3.49
	9	saturated to unsaturated ratio (15086)	10.58	*	B6	1.05
	12	total fat (15084)	21.47	**	B6	236.62
	12	saturated fat (15083)	21.47	**	B6	68.65
	12	unsaturated fat (15082)	21.24	**	B6	1.51
	12	acyl chain length (15085)	16.27	*	B6	0.40
	12	collagen (15089)	22.09	**	B6	0.42
	12	collagen integrity (15090)	17.65	**	D2	4.16
	12	lipid to protein ratio (15087)	20.95	**	B6	99.76
	17	lipid to protein ratio	14.27	*	B6	86.64
		(15087)	13.69	*	B6	85.26
Liver	5	total fat (15092)	12.49	*	B6	0.30
	5	saturated fat (15091)	11.73	*	B6	0.15
	9	collagen (15096)	14.48	*	B6	0.10
			12.31	*	B6	0.10
	9	collagen integrity (15097)	13.28	*	D2	76.10
			11.69	*	D2	73.89
	17	total fat (15092)	16.72	*	B6	0.35
	17	saturated fat (15091)	13.84	*	B6	0.17
	17	acyl chain length (15093)	12.55	*	B6	1.17
	17	lipid to protein ratio	13.04	*	B6	0.05
		(15094)	19.36	**	B6	0.06
	18	glycogen (15098)	10.9	*	D2	0.22
Muscle	5	collagen integrity (15105)	11.72	*	B6	20.77
	9	collagen (15104)	17.33	*	D2	1.03
	9	collagen integrity	17.19	*	B6	23.45
		15105)	16.81	*	B6	23.60
	9	Glycogen (15106)	12.31	*	B6	0.27
	15	collagen (15104)	11.1	*	D2	0.86

The degree of LRS significance was denoted as: suggestive*, significant**. Genome-wide significance (p<0.05) and suggestive (p<0.63) thresholds were calculated as likelihood ratio statistic (LRS) in linkage analyses computed for 1000 permutations [60]. The values of total, unsaturated and saturated fat, the ratio of saturated to unsaturated fat, collagen and collagen integrity, the ratio of lipid to protein and glycogen are relative values as described in experimental procedures. GeneNetwork Record ID was given in parenthesis for each trait.

ISSN: 1471-2164