Pyrosequencing analysis of the human microbiota of healthy Chinese undergraduates

BMC Genomics

Table 1 Comparison of phylotype coverage and diversity estimation of the 16S rRNA gene libraries at 3% dissimilarity from the pyrosequencing analysis

Group	Reads	OTUs ¹	Good’ s ²	ACE	95% C. I.		Chao 1	95% C. I.		Shannon	Simpson	Evenness ³
Nasopharynx	30521	998	0.969	2290.3	2142.5	2457.3	1802.0	1622.9	2032.6	4.0960	0.0945	0.0117
Saliva	28309	1129	0.962	2431.9	2286.5	2595.7	1907.9	1742.1	2118.6	4.6437	0.0780	0.0131
Dominant hand	43708	1768	0.960	3993.1	3796.7	4208.6	3082.0	2853.2	3359.2	5.2870	0.0297	0.0279
Feces	54179	1959	0.965	3967.6	3787.5	4165.7	3233.9	3019.2	3492.5	5.4907	0.0197	0.0349

¹The operational taxonomic units (OTUs) were defined with 3% dissimilarity level.
²The coverage percentage (Good’s), richness estimators (ACE and Chao1) and diversity indices (Shannon and Simpson) were calculated using Good’s method [66] and the mothur program, respectively.
³The Shannon index of evenness was calculated with the formula E = H/ln(S), where H is the Shannon diversity index and S is the total number of sequences in that group.

ISSN: 1471-2164