Sequence analysis of BnaMPKs. (A) Partial amino acid alignment of the 12 BnaMPK members. Multiple sequence alignment was performed using the ClustalX1.83 and illustrated by BOXSHADE (http://www.ch.embnet.org/software/BOX_form.html). Identical amino acids are shaded in black, and similar amino acids are shaded in gray. The phosphorylation-activation motif (TDY and TEY) was highlighted by the box and the PK sub-domains of VII and VIII were labelled as above. (B) Schematic diagram of amino acid motifs of BnaMPKs. Motif analysis was determined by using MEME4.0 program as described in Methods. Different colors of the boxes represent 10 motifs in the corresponding position of each BnaMKK proteins. The information of 10 motifs was illustrated in Additional file 7: Figure S5.