Figure 5
From: Identification and analysis of MKK and MPK gene families in canola (Brassica napusL.)
Subcellular localization of three BnaMKK, four BnaMAPK proteins and BnaWRKY20 in N. benthamiana cells using green fluorescence protein (GFP). Panels A-E represent BnaMKK2-sGFP (A and B) BnaMKK3-sGFP (C), BnaMKK4-sGFP (D and E) and Panels F-M represent the subcellular localization of BnaMPK3-sGFP (F and G), BnaMPK5-sGFP (H), BnaMPK6-sGFP (I and J), BnaMPK9-sGFP (K and L) and BnaWRKY20 (M) respectively. Panels B, E, G, J, and L are subcellular localization of BnaMKK2, -4, and BnaMPK3, -6, -9 under mannitol treatment (500 mM for 1 h). In each panel, the extreme left is GFP fluorescence, the middle bright field and the right an overlay of the two images. Bar = 50 μm.