Subcellular localization of three BnaMKK, four BnaMAPK proteins and BnaWRKY20 in N. benthamiana cells using green fluorescence protein (GFP). Panels A-E represent BnaMKK2-sGFP (A and B) BnaMKK3-sGFP (C), BnaMKK4-sGFP (D and E) and Panels F-M represent the subcellular localization of BnaMPK3-sGFP (F and G), BnaMPK5-sGFP (H), BnaMPK6-sGFP (I and J), BnaMPK9-sGFP (K and L) and BnaWRKY20 (M) respectively. Panels B, E, G, J, and L are subcellular localization of BnaMKK2, -4, and BnaMPK3, -6, -9 under mannitol treatment (500 mM for 1 h). In each panel, the extreme left is GFP fluorescence, the middle bright field and the right an overlay of the two images. Bar = 50 μm.