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Table 1 BnaMPKs and BnaMKKs identified in this study

From: Identification and analysis of MKK and MPK gene families in canola (Brassica napusL.)

Gene

GenBank Acc No.a

EST count

AA No.

pI value

Arabidopsis orthologb/AGI No.

Rice orthologb/locus

Subcellular localizationc

 
 

PSORT

CELLO

ESLPred

experimental

BnaMKK1

JQ708028

3

356

6.75

AtMKK1/ At4g26070

OsMKK1/Os06g05520.1

nuc

nuc

cyt

 

BnaMKK2

JQ708029

3

365

5.99

AtMKK2/ At4g29810

N/A

cyt

cyt, nuc

cyt

cyt, nuc

BnaMKK3

JQ708030

3

519

5.58

AtMKK3/ At5g40440

OsMKK3/Os06g27890.1

cyt, nuc

cyt, pm

cyt

cyt, nuc

BnaMKK4

JQ708031

4

357

9.2

AtMKK4 /At1g51660

OsMKK5/Os06g09180.1

nuc, cyt

nuc

nuc

cyt, nuc

BnaMKK5

KC246595

2

333

8.89

AtMKK5/ At3g21220

OsMKK4/Os02g54600.1

mit

nuc

nuc

 

BnaMKK6

JQ708032

2

357

6.19

AtMKK6/ At5g56580

OsMKK6/Os01g32660.1

cyt

nuc, cyt

cyt

 

BnaMKK8

No

1

  

AtMKK7/ At5g56581

     

BnaMKK9

JQ708033

1

307

8.22

AtMKK9/ At1g73500

N/A

chl, nuc

mit

cyt

 

BnaMPK1

JQ708034

2

371

6

AtMPK1/ At1g10210

OsMPK7/Os06g48590.1

nuc

cyt

cyt

 

BnaMPK2

JQ708038

5

374

6.6

AtMPK2/ At1g59580

N/A

  

cyt

 

BnaMPK3

JQ708040

4

371

6

AtMPK3/ At3g45640

OsMPK3/Os03g17700.1

nuc, cyt

cyt

nuc

cyt, nuc

BnaMPK4

JQ708041

7

374

6.06

AtMPK4/ At4g01370

OsMPK4/Os10g38950.1

cyt

cyt

nuc

 

BnaMPK5

JQ708042

3

374

5.7

AtMPK5/ At4g11330

OsMPK2/Os08g06060.1

cyt

cyt

nuc

cyt, nuc

BnaMPK6

JQ708043

6

396

5.19

AtMPK6/ At2g43790

OsMPK6/Os06g06090.1

nuc

cyt

nuc

cyt, nuc

BnaMPK7

No

13

  

AtMPK7/ At2g18170

     

BnaMPK8

JQ708044

12

582

6.65

AtMPK8/ At1g18150

N/A

nuc

cyt

nuc

 

BnaMPK9

JQ708045

1

502

8.42

AtMPK9/ At3g18040

OsMPK17-1/Os06g49430.1

cyt, nuc

cyt

cyt

cyt, nuc

BnaMPK10

No

2

  

AtMPK10/At3g59790

     

BnaMPK12

No

4

  

AtMPK12/At2g46070

     

BnaMPK13

No

1

  

AtMPK13/At1g07880

     

BnaMPK15

No

1

  

AtMPK15/At1g73670

     

BnaMPK16

JQ708035

12

559

8.8

AtMPK16/At5g19010

OsMPK/Os05g05160.1

cyt

cyt

cyt

 

BnaMPK17

JQ708036

10

488

6.83

AtMPK17/At2g01450

N/A

cyt

cyt

cyt

 

BnaMPK18

No

32

  

AtMPK18/At1g53510

     

BnaMPK19

JQ708037

7

600

9.2

AtMPK19/At3g14720

N/A

cyt

pp,cyt

cyt

 

BnaMPK20

JQ708039

10

612

9.23

AtMPK20/At2g42880

OsMPK20-1/Os01g43910.1

cyt

cyt

mit

 
  1. aThe full-length cDNA was cloned and deposited in GenBank. No, cloning not sucessful.
  2. bPutative orthologs were identified by InParanoid (http://inparanoid.sbc.su.se/cgi-bin/index.cgi) with a maximum score. N/A, homolog but not ortholog was identified.
  3. cSubcellular localizations are predicted using three different programs, one is PSORT (http://psort.nibb.ac.jp), the second is CELLO v2.5 (http://cello.life.nctu.edu.tw) and the third is ESLPred (http://www.imtech.res.in/raghava/eslpred/index.html). nuc-nuclear, cyt-cytoplasm,pp-perplasm, mit-mitochondria,chl-chloroplast,and pm-plasma membrane.
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BMC Genomics

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