Predicted amino acid sequences of the Acropora GiMAP proteins responding to challenge with the bacterial cell wall derivative MDP. The figure shows the proteins encoded by Cluster012256 (GiMAP1), Cluster024980 (GiMAP2) and Cluster015015 (GiMAP3) from the A. millepora transcriptome assembly, aligned with their putative orthologs from the related species A. digitifera (aug_v2a23822t1, aug_v2a23824t1 and aug_v2a23823t1 respectively). Boxed regions correspond to conserved structural features of the AIG domain (cd01852; pfam04548) based on alignment of the coral sequences with the 2XTM_A (GDP-bound human GiMAP2) and 3LXX_A (human GiMAP4) crystal structures using Cn3D v4.1 . Note that in the case of GiMAP1_Am, the protein predicted from transcriptome Cluster012256 terminates at the “X” at position 223 indicated by the arrow, and inspection of individual Illumina reads did not resolve the ambiguity. However, extending the translation resulted in a further 9 AA region matching the A. digitifera presumed ortholog and GiMAP2 sequences in both species.