Figure 6

TSA or 5Aza treatment does not have an effect on cubilin monoallelic expression in PRTCs. A and B, qPCR analysis of cubilin and megalin mRNA expression in RNA isolated from PRTCs treated for 7 days with DMSO or with the indicated doses of TSA. C, shows immunoblot analysis of extracts of PRTCs treated for 7 days with vehicle or varying concentrations of TSA or 5Aza using antibodies to cubilin, megalin, and actin. D shows the number of anti-EGFP positive cells as a fraction of total cells counted in 21 imaged fields of DMSO-treated and 34 imaged fields of 10 nM TSA-treated PRTCs from three experiments. Asterisks indicate that differences between vehicle control and drug treatment groups were significant at p<0.05.