Diversity of the accessory genome. As examples, BLAST alignments of de novo assembled not-in-reference accessory genome contigs from all three clone CHA isolates to the PA14 Region of Genome Plasticity (RGP) 26 (panel A) and the PAGI-2 genomic island (panel B) are shown. Contigs from CHA are coloured black, those from PT22 are red and contigs from 491 are grey, while the dark blue boxes represent the annotated ORFs from the P. aeruginosa PA14 genome and the PAGI-2 genomic island, respectively. For details on the annotated ORFs, please refer to the respective original publications ( for RGP26 from PA14,  for PAGI-2). PT22 harbours a complete copy of the PAGI-2 island, while 491 has a partial copy and it is absent in CHA. Figures were produced using the R package Genomegraphs .