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Table 5 Accessory DNA elements from other P. aeruginosa genomes detected in strains CHA, PT22, and 491

From: Intraclonal genome diversity of Pseudomonas aeruginosa clones CHA and TB

From defined genomic islands

Name

No. of ORFs

Present parts

PAGI-2 (RGP29)

111

strain PT22: complete 105 kb island (> 99.9%); strain 491: ORFs C1-4; C36–111 (80 – 100%)

PAGI-5 (RGP7)a

121

strain 491: complete 99.4 kb island (> 99.2%)

PAGI-6 (RGP87)

47

phage CTX-like ORFs 6PG1–28 and 6PG32–38 (86.8 – 100%)

PAGI-8 (RGP62)

12

ORFs 8PG1; 8PG7-8 (85.6 – 95.2%)

pKLC102 (RGP7)a

105

strain CHA: ORFs CP1–3; CP12–14; CP27; CP30-31; CP34–44; CP50–54; CP57–83; CP87–89; CP102-103 (94.8 – 99.3%); strain PT22: ORFs CP1–3; CP9–14; CP18–21; CP26–27; CP29–30; CP33–45; CP47–56; CP58–83; CP87–93; CP102-103 (84.3 – 99.5%)

LESGI-2 (RGP85)

18

complete 31.7 kb island (98.8 – 100%)

LESGI-3 (RGP27)

115

strain PT22: complete 110.6 kb island (90.4 – 100%); strain 491: PLES_26051–26061; PLES_26211–26221; PLES_26421–27102 (81.7 – 100%)

LESGI-4 (RGP23)b

31

complete 39.4 kb island (97.4 – 100%)

LES-prophage 1 (RGP3)

19

complete 14.8 kb island (81.6 – 100%)

LES-prophage 3 (RGP82)

51

strain 491: homologs to 18 ORFs (88.3 – 98.4%)

LES-prophage 6 (RGP10)

12

strains CHA and 491: PLES_41181 – 41241 (90.7 – 100%); PLES_41191 only partially covered

From other RGP insertions

RGP (host strains)

No. of ORFs

Present parts

RGP6 (2192)

41

PA2G_05961-05962 (> 99.7%)

RGP9 (2192)

14

PA2G_00059-00065; PA2G_00072 (95.1 – 100%)

RGP26 (PA14)

39

strain CHA: PA14_30960; PA14_31070– 31150 (84.8 – 95.2%); strain PT22: PA14_30850–30960; PA14_31070–31200 (81.1 – 98.6%); strain 491: PA14_30850–30970; PA14_31110–31250 (79.7 – 97.2%)

RGP27 (PACS2)

74

strain 491: PAERPA_01003080–3085; PAERPA_01003110; PAERPA_01003119–3120; PAERPA_01003136–3154 (84.5 – 100%)

RGP29 (PACS2)

10

complete RGP-insertion (98.5 – 100%)

RGP35 (2192)

43

strain 491: PA2G_02937–2942; PA2G_02953; PA2G_02956–02957; PA2G_02961–02963; PA2G_02965; PA2G_02969; PA2G_02972-02973 (92.2 – 100%)

RGP36 (PA14)

31

PA14_15620-15630; PA14_15650-15660 (96.4 – 99.7%)

RGP42 (2192)

11

strain CHA: PA2G_05286-05290 (97.1 – 99.5%); strain 491: PA2G_05286–05292 (95.4 – 100%)

RGP42 (PA7)

54

strain PT22: PSPA7_5339-5340 (85.1 – 89.1%)

RGP63 (PA7)

72

strain PT22: PSPA7_0075 (86.3 – 90.5%); PSPA7_0108-0114 (> 99.9%)

RGP77 (PA7)

53

strain CHA: PSPA7_3708; PSPA7_3723; PSPA7_3726-3734 (83.4 – 93.3%); strain PT22: PSPA7_3696-3708; PSPA7_3723; PSPA7_3726–3735; PSPA7_3738-3747 (79.5 – 100%); strain 491: PSPA7_3696-3708; PSPA7_3723; PSPA7_3726–3729; PSPA7_3731-3733; PSPA7_3738-3740; PSPA7_3747 (79.3 – 100%)

  1. Present parts printed in bold are conserved in all three clone CHA strains. Pairwise % nucleotide identity of the corresponding sequence contigs is given in brackets.
  2. a majority of assigned contigs mapped on both PAGI-5 and pKLC102 references which share a large set of highly homologous genes.
  3. b contigs also mapped on island PAGI-1, a variant of LESGI-4.