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Table 1 AMBIENT modules differentially regulated in response to nitrogen stress

From: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules

Module

q-value

No. of reactions

No. of metabolites

Reactions with associated genes

Specific function(s)

Up

  u1

<0.004

8

2

8

Nitrate/nitrite uptake and assimilation

  u2

0.00

3

2

3

Cytosine/uracil uptake and assimilation

  u3

0.02

6

2

6

Ethanolamine uptake and assimilation

  u4

0.02

4

2

4

Arginine/ornithine metabolism

  u5

0.02

4

3

4

Histidine degradation

  u6

0.01

1

1

1

Arginine metabolism

  u7

0.02

1

1

1

Ammonium transport

  u8

0.02

4

1

4

Cation transport

  u9

0.02

1

1

1

Cyanate degradation

  u10

0.02

5

4

5

Fatty acid metabolism

  u11

0.02

1

1

1

Hydrogen peroxide metabolism

  u12

0.02

1

1

1

Hydrogen peroxide metabolism

  u13

0.02

1

1

1

Hydrogen peroxide metabolism

  u14

0.03

3

2

3

Energy metabolism

  u15

0.03

2

1

2

Thiamine transport and assimilation

  u16

0.04

4

1

4

Galactose metabolism

  u17

0.03

1

1

1

Allantoin transport

  u18

0.03

1

1

1

Hydrogen peroxide metabolism

  u19

0.04

3

1

3

Gluconate degradation

  u20

0.04

2

1

1

Fatty acid metabolism

  uHP

<0.004

4

1

4

Hydrogen peroxide metabolism

Down

  d1

<.004

152

75

110

LPS, RNA and DNA synthesis, central carbon metabolism

  d3

0.04

5

3

5

Pantothenate metabolism

  d4

0.04

4

2

3

Fatty acid metabolism

  d5

0.04

4

3

0

Isoprenoid utilization

  d6

0.05

4

2

3

Fatty acid metabolism

  d7

0.04

3

2

2

Xylose uptake and assimilation

  1. Up- and down-regulated modules found using AMBIENT in nitrogen stress, in decreasing order of score, indicating the number of reactions and metabolites present in each. Scores are calculated by AMBIENT, taking into account the logarithm of the fold-change of the genes associated with each reaction and the connectivity in the metabolic network of each metabolite in the module. The number of genes associated with these reactions according to the Model SEED M. smegmatis model is also shown. Functional assignments are based on manual inspection of the exact function of the member reactions of the modules. Diagrams for each module are available as Additional files 3 (Up modules) and 4 (down modules), except for u11, u12, u13 and u18, which are combined in uHP.