Figure 2

Map of the genomic rearrangement in the 46’ region of the BW4005 chromosome. The top map shows both the ~180 kb duplication of the ancestral chromosomal region, that extends between the two IS5 elements located at positions 1,993,429 and 2,172,740, and the relevant IS3 elements contained within that duplication. The copy on the right carries the 2668-bp deletion containing part of ogrK and all of yegR and yegS. The bottom line shows the restriction map derived from the PFGE profile of XbaI-digested genomic DNA of BW4005.2. Vertical arrows indicate locations of XbaI sites and horizontal lines with arrows indicate the locations of primers used for the identification of the new junction generated by the duplication (shown in the box). The sizes of the different XbaI fragments are indicated between the vertical arrows. For gel documentation of the BW4005.2 PFGE profiles showing the extra XbaI bands of 61.5 and 19 kb (indicated by dashed lines), see Figure 1d,e. Genome positions of XbaI restriction sites are based on the genome sequence of BW2952 [29].