Morphological and molecular confirmation of N. apis species status. (a) Light micrograph of N. apis spore. Scale bar: 10 μM. (b) Agarose gel electrophoresis of PCR products showing the presence of a single N. apis infection in the DNA sample. The presence of a single N. apis infection in the DNA sample intended for 454 pyrosequencing was confirmed by species-specific PCR and further sequencing analysis.