Figure 5

Sex-specific sequences in sablefish. A) The major features of the sablefish sex region, including the relative location of the X and Y-specific insertions to the start of the gsdf coding region and the location of the subsequent PCR reaction primers B) PCR reaction showing the amplification of a single, larger fragment in 8 unrelated female fish, and two fragments in the males with the Y-fragment 412bp smaller in size C) Nested PCR demonstrating the presence of the Y-specific insertion solely in the males. D) time-series of pre-hatch and post-hatch sablefish showing genetic sex-identification based on the X-specific insertion, and gsdf expression in these individuals. For B), C) and D) “L” represent the 1kb o’generuler plus ladder (Thermo Scientific), with the brightest two bands at 500 and 1500bp, “N” represents the negative control.