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Table 1 Comparison of Illumina and modified method

From: Multiplexed Illumina sequencing libraries from picogram quantities of DNA

Parameter Illumina method Drawback Modified method Benefit
Adapters Indexed Entire sample receives one barcode Universal Flexibility; multiple barcodes can be added later
Size selection Gel-mediated Time-consuming; sample loss No size selection Time savings; sample retention
Amplification 10-18 cycles Potentially under-optimized Monitored by qPCR Stop cycling during log growth phase
All steps Constrained by kit Difficult to modify Transparent and kit-independent Flexibility