Figure 4

Scatterplot of DNA methylation (x-axis) and gene expression (y-axis) differences between HPVEC and HPAEC for genes showing reciprocal methylation/gene expression change. Gates were set at the limits of technical noise (methylation +/− 10%, gene expression +/− 0.5 fold change). Each set of HM450 probe datapoints (x-axis) with a common set of gene expression values (y-axis) represents a gene likely to be under epigenetic regulation by DNA methylation with decreased methylation associated with elevated gene expression in HPAEC relative to HPVEC (A) and vice versa (B). Genes highlighted in red included NOS3 and vWF. Note that in many instances, genes contain multiple HM450 probes for each gene expression measure.