Table 2 DNA Methylation of probes located near transcription start sites of selected genes associated in endothelial function and corresponding gene expression levels
Selected genes showing changes in both promoter methylation and gene expression | ||||
|---|---|---|---|---|
Gene symbol | Description | Gene expression LogFC (HPAEC ‒ HPVEC | Average β of HPAEC ‒HPVEC | Probes located in promoter region / total number of probes* |
NOS3 | Nitrix Oxide Synthase | -1.233 | 0.314 | 6/28 (21%) |
vWF | von Wildebrand Factor | -2.523 | 0.124 | 4/38 (11%) |
MGP | Matrix Gla protein | -3.202 | 0.225 | 1/3 (33%) |
GJA5 | Connexin40 | -3.434 | 0.122 | 7/15 (47%) |
APOLD1†| apolipoprotein L domain containing 1 [Homo sapiens] | -0.061 | 0.684 | 7/32 (22%) |
HIF3A†| Hypoxia Inducible Factor 3, alpha subunit | -0.599 | -0.161 | 8/23 (35%) |
EphB1†| Ephrine type-B receptor 1 | 1.050 | 0.284 | 2/36 (6%) |
ANGPTL†| Angiopoietin-like 2 | 0.768 | -0.178 | 2/6 (33%) |
SELE†| E-Selectin | 0.934 | 0.165 | 2/3 (67%) |
FLT1 (VEGFR1) †| Vascular Endothelial Growth Factor Receptor 1 | 0.930 | 0.333 | 3/28 (11%) |
COL6A3 | Collagen, type VI, alpha 3 | 4.117 | -0.128 | 9/53 (17%) |
HSD11B1 | Hydroxysteroid (11-beta) Dehydrogenase 1 | 1.092 | -0.177 | 8/9 (89%) |
VEGFC | Vascular Endothelial Growth Factor C | 0.662 | -0.101 | 5/13 (38%) |
HGF | Hepatocyte Growth Factor (hepapoietin A; scatter factor) | 3.973 | -0.092 | 7/10 (70%) |
ANGPT1 | Angiopoietin 1 | 1.172 | -0.210 | 2/16 (13%) |
Genes showing expression changes but no changes in DNA methylation | ||||
Gene symbol | Description | Gene expression LogFC (HPAEC ‒ HPVEC | Average β of HPAEC ‒HPVEC | Probes located in promoter region / total number of probes* |
ARG2 | Arginine 2 | 0.445 | <0.001 | 11/15 (73%) |
DLL1 | Delta like protein 1 precursor | 0.077 | 0.005 | 12/38 (32%) |
JAG1 | Jagged 1 precursor | 0.605 | <0.001 | 10/24 (42%) |
JAG2 | Jagged 2 precursor | 0.369 | <0.001 | 8/47 (17%) |
KDR (VEGFR2) | Kinase Insert Domain Receptor | 1.552 | 0.003 | 9/16 (56%) |
HEY2 | hairy/enhancer‒of‒split related with YRPW motif 2 | 1.506 | <0.001 | 11/21 (52%) |
NOTCH | neurogenic locus notch homolog protein 4 preproprotein | 0.429 | 0.024 | 3/160 (2%) |
EphB2 | neurogenic locus notch homolog protein 4 preproprotein | 0.262 | 0.007 | 5/46 (11%) |
NRP1 | Neuropilin 1 | 0.529 | 0.002 | 6/29 (21%) |
EphB4 | Ephrine type‒B receptor 4 | 0.732 | <0.001 | 7/29 (24%) |
EphB4 | Intercelluar adhesion molecule‒2 | 0.808 | 0.029 | 11/15 (73%) |