Figure 1

Growth and sampling of cells during the stress and adaptation growth regime and choice of histone acetylation marks for study. A Growth curves of cells during the stress and recovery growth regime. Growth curves for two independent experiments show cell growth, the dilution of culture into and out of KCl (1M) containing media and points at which samples (A-E) were removed for analysis by genome-wide gene expression profiling and ChIP-on-chip analysis. Cells were maintained at a density between 10⁶/ml and 2×10⁷/ml throughout. B-C Average levels of Gcn5 and acetylated lysine residues in histones for 5 groups of genes with different characteristic Gcn5 intra-genic distributions (the 5 gene clusters are based on previous publication [7], which are either high at ORF (cluster 1), low at ORF (cluster 2), high at both ORF and IGR (cluster 3), high at 3′IGR (cluster 4), or high at 5′IGR (cluster 5)). Gcn5 and histone acetylation levels for this 5 gene clusters are plotted separately for ORF (B) and IGR (C) regions.