Verification of detected CNVs by qPCR. The horizontal line represents the relative quantification level 1. Two copies are shown by squares, one copy by triangles, zero copies by circles and three copies by rhombus. (A) The CNV region on ECA1 at 155.63 Mb was validated in nineteen horses (solid symbols). One horse showed a homozygous deletion instead of two copies (unfilled symbol). (B) The CNV region on ECA1 at 156.69 Mb was validated in all twenty horses (solid symbols). (C) The CNV region on ECA8 at 4.50 Mb was validated in sixteen horses (solid symbols). Four horses showed different copies as detected by SNP chip analysis (unfilled symbols).