Increased N50 transcript length and number of predicted transcripts in the ASU annotation. A. The distribution of transcript lengths is shown for the ASU, NCBI and Ensembl genome annotations. The ASU annotation transcript N50 length of 5,355 bp is greater than values for the first generation annotations from Ensembl (2,037 bp) and NCBI (2,364 bp). B. A boxal plot showing the median (horizontal line) and boundaries for the 25th and 75th percentiles (box) as well as the range for the ASU, NCBI, and Ensembl predicted transcripts. C. The Notch ligand dll1 is an example of gene whose annotation has been markedly improved in the ASU annotation.