Figure 1

Co-treatment of HMCs with TGFβ and CCN2 inhibits cell migration in an ERK dependent manner. A. HMCs were grown to confluence, a wound scratch made and cell migration assessed in response to treatment with TGFβ, CCN2 or both together. Both TGFβ and CCN2 increased cell migration, but together inhibited the migration of cells. B. Altered migration was not dependent on cell proliferation, as assessed by MTT uptake. C. Inhibition of MEK/ERK signalling with the inhibitor PD-98059 was sufficient to restore migration in cells co-treated with TGFβ and CCN2. D. This effect was not dependent on altered cell proliferation. Data representative of n = 3 independent experiments with 10 biological replicates per condition per experiment. **p < 0.01.