Procedures | Output |
---|---|
5′-P independent cloning (5′CIP+PNK method) | Rahman total RNA, size fractionation (15-30nt) library |
Total reads by Pyrosequencing | 151,656 reads |
Primer trimming, size-limiting (15–40nt) | 140,571 reads |
Unique sequences (percentage) | 98,414 unique sequences (82,780 cloned only once; 84.1%) (15,634 cloned more than once; 15.9%) |
Scan for tRNA, rRNA | tRNA: 517 sequences rRNA:1,753 sequences |
Scan for SINE/LINE, EhERE elements | 4,439 sequences |
Map to E. histolytica genome | 52,028 sequences |
Map to E. histolytica predicted ORFs | 35,157 sequences |