Figure 5

H4K5ac in the presence of TF binding sites relative to the TSS. (A) Percent of expressed genes acetylated for H4K5 by CFC with (red) or without (black) a TFBS at that position in the promoter, 2 kb upstream of the TSS in 5 bp increments. Lines are smoothed with a running median of width 10. Given that a gene can be acetylated at multiple sites in the promoter regardless of whether it is expressed or not, the percentage of acetylated genes at a specific position can be greater than 100%. Percent of unexpressed genes that are acetylated are plotted in gray. (B) Average read coverage of H4K5ac by CFC, ± 2 kb relative to the TSS, for all genes (black), genes with a TFBS present 500 bp (orange), 800 bp (green), or 1100 bp (turquoise) upstream of the TSS, and genes with no TFBS upstream of 100 bp of the TSS (blue). The relative contribution of gene clusters for (C) H4K5ac by CFC, (D) H4K12ac by CFC, and (E) mock IgG-IP with either no TFBS present in the promoter, or no TFBS, one TFBS, and multiple TFBS present upstream of 150 bp relative to the TSS. Clusters are defined in Figures 4A, 4C, and 4E, respectively.