Figure 1From: MHC genotyping of non-model organisms using next-generation sequencing: a new methodology to deal with artefacts and allelic dropout454 reads filtering: pyrosequencing data quality assessment. Pathway illustrating initial filtering steps to ensure data quality of reads obtained by 454 pyrosequencing and to facilitate the subsequent allele and artefact identification workflow. The number of reads included in a filtering step is indicated on the left of each arrow and the percentage of the initial number of reads in brackets. The number of discarded reads is shown on the right of each arrow.Back to article page