Figure 1

Paired–end RAD Sequencing Overview. A. Genomic DNA is digested with a restriction endonuclease. B. After ligation with a primary adapter, the fragments are sheared, then ligated with a secondary adapter. C. A composite mixture of variable length fragments is recovered from each restriction enzyme digestion site. These fragments are size selected, amplified and sequenced on a next-generation DNA sequencing platform using paired end chemistry. D. Development of the genomic assembly around each digestion site is then completed bioinformatically.