Figure 3

PCA of the expression level of 579 lipid genes in wild type and hepatic RXRα-KO mice treated with and without RA. Wild type and hepatic RXRα KO mice were treated with and without RA for 7 days (150 mg/kg diet, n=3). The expression of 579 genes involved lipid homeostasis was studied. (A) Score plot of PCA showing the difference among the groups. Spots within an ellipse belong to the same group. Arrows represent the direction separating groups from the wild type (WT) mice (open square □). There is no significance in the C1component observed across all groups. (B) A histogram that shows the loading value of genes on C2. Genes with high loading value (≥ 0.5) on C2 had higher mRNA levels in RXRα KO than wild type livers. In contrast, genes with low loading value (≤ -0.5) on C2 had increased mRNA levels due to RA treatment.