Figure 1

Quantification of P. aeruginosa PAO1 gene expression. A. Gene expression profile across the entire P. aeruginosa PAO1 genome (6.3 Mb). Each column represents one of the 5570 annotated genes in the PAO1 genome, with the x-axis showing gene order (from the DNA replication origin), and the y-axis showing the log₁₀ of transcript abundance (RPKM values) for each gene. The arrows point to a few of the genes or gene clusters that are highly expressed. PA0456: probable cold-shock protein; PA1804: DNA-binding protein HU; PA2742: 50S ribosomal protein L35; PA2966: acyl carrier protein; conserved hypothetical protein; fliC: flagellin type B; pilA: type 4 fimbrial precursor; oprF, oprG, oprI: outer membrane proteins; azu: azurin precursor. B. 56.6 kb genome region (PA1857 to PA1938) that shows low levels of gene expression (log (RPKM + 1) < 2). C. 33.8 kb genome region (PA4235 to PA4277) that exhibits high levels of gene expression (log (RPKM + 1) > 2). D. Distribution of the transcript abundance of the 5570 annotated genes in the PAO1 genome, with the x-axis showing gene number (sorted from high to low values), and the y-axis showing the log₁₀ of transcript abundance for each gene in the wild type. The dashed lines indicate gene numbers where mean and medium values are first detected.