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Table 2 Bacterial strains and plasmids used in this study

From: Transcriptome profiling reveals links between ParS/ParR, MexEF-OprN, and quorum sensing in the regulation of adaptation and virulence in Pseudomonas aeruginosa

Strains and plasmids

Relevant charactersa

Reference or source

P. aeruginosa

  

PAO1

wild-type (WT)

[51]

∆parS

PAO1 Tn5 mutant, insertion at bp 402 in parS, GnR

[51]

∆parR

PAO1 Tn5 mutant, insertion at bp 299 in parR, GnR

[51]

E. coli

  

DH5α

F-recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ (argF-lacZYA)I169 ϕ 80lacZ ΔM15λ-

GIBCO-BRL

Plasmids

  

pPROBE-KT2

KmR, GFP based promoter trap vector containing a promoter-less gfp gene

[58]

pKT2-parSR

2.3 kb DNA fragment containing parS and parR genes in pPROBE-KT2

This study

  1. aKmR and GnR = kanamycin and gentamycin resistance, respectively.