Strains and plasmids | Relevant charactersa | Reference or source |
---|---|---|
P. aeruginosa | Â | Â |
PAO1 | wild-type (WT) | [51] |
∆parS | PAO1 Tn5 mutant, insertion at bp 402 in parS, GnR | [51] |
∆parR | PAO1 Tn5 mutant, insertion at bp 299 in parR, GnR | [51] |
E. coli | Â | Â |
DH5α | F-recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ (argF-lacZYA)I169 ϕ 80lacZ ΔM15λ- | GIBCO-BRL |
Plasmids | Â | Â |
pPROBE-KT2 | KmR, GFP based promoter trap vector containing a promoter-less gfp gene | [58] |
pKT2-parSR | 2.3Â kb DNA fragment containing parS and parR genes in pPROBE-KT2 | This study |