Examples of P. acnes small RNAs. (A) and (B) show the transcription and structure, respectively, of the 5′ UTR of PPA0166, which encodes a putative L-lactate permease. Alternative folding is shown bottom right of (B). (C), (D), and (E) correspond to examples of potential antisense RNAs that bind 5′ RBSs, internal RBSs, and sites internal to the coding region, respectively. (F) shows an example of highly abundant sRNA that does not appear to overlap mRNA. For each of these panels, the tracks show the positions of annotated genes, the position of TSSs (Additional file 3) and the gRNA-seq data for both strands (black positive values correspond to the forward strand, while red negative values correspond to the reverse strand). The remainder of the labelling is as Figure 2.