Leaderless mRNAs and transcripts of genes requiring reannotation. (A) and (B) correspond to examples of leaderless mRNA. PPA0769 encodes pyruvate kinase, and PPA2225 ferritin. (C) and (D) correspond to examples of genes requiring reannotation. PPA0885 encodes hydroxyethylthiazole kinase, and PPA0228 a putative methyltransferase. For each of these panels, the tracks show, from top to bottom, the positions of annotated genes, the position of TSSs (Additional file 3), gRNA-seq data, and BlastP hits or operon predictions. The remainder of labelling is as Figure 2. (E) shows the transcription data for eno (PPA0545), which encodes phosphopyruvate hydratase. Purple boxes indicate computational predictions of the operon structure in this region . As in previous figures, all the genes are drawn left to right with their corresponding RNA-seq data (black positive values for forward strand, red negative values for the reverse strand).