Location of 3′ tRNA processing sites. (A), (B), (C) and (D) correspond to tRNAs Ala (GGC, PPA2421), Gly (CCC, PPA2455), Ser (TGA, PPA2461) and Gln (TTG, PPA2415), respectively. As described for Figure 2, all the corresponding genes are shown left to right with the RNA-seq data for genes on the forward and reverse strands depicted by black, positive bars and red, negative bars, respectively. For each panel, the tracks depict, from top to bottom, the location of the TSS, the position of the tRNA gene, the gRNA-seq data, and the average of the dRNA-seq values before and after TAP treatment (combining values for the control and potassium-downshift sample). Vertical (double-headed) arrows indicate the peaks that correspond to processing on the 3′ side of the tRNAs. The sequences flanking these sites are also shown. The remainder of the labelling is as Figure 2. In (A) and (B), the dRNA-seq reads corresponding to the TSSs are too low to be detected at the scales used to identify the processing sites.